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KMID : 0545120040140061324
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Journal of Microbiology and Biotechnology
2004 Volume.14 No. 6 p.1324 ~ p.1326
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Lack of O-Polysaccharide Renders Bradyrhizobium japonicum More Resistant to Organic Acid Stress
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Oh ET
Ju YJ/Koh SC/Kim YH/Kim JS/So JS
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Abstract
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In previous studies we isolated an isogenic LPSmutant of Bradyrhizobium japonicum 61A101C which was completely devoid of O-polysaccharide and had altered cell surface characteristics. Subsequently the mutated gene was identified cloned and used to complement the LPS- mutant strain JS314 to restore the phenotype. Since it has been reported that in Escherichia coli LPS O-polysaccharide is involved in resistance to an organic acid such as acetic acid under low pH (Barua et al. Molecular Microbiology 43: 629- 640 2002) we compared the organic acid resistance of the three B. japonicum strains; wild-type 61A101C the LPSmutant JS314 and the complemented strain to determine whether the role of O-polysaccharide in the resistance to organic acid could be generalized. Growth of all three strains was inhibited by the presence of 3 mM acetic acid under acidic condition (pH 5.5). To our surprise however in the presence of 2 mM acetic acid wild-type and the complemented strains did not grow while the LPS- mutant showed a significant growth. Therefore unlike in E. coli the lack of Opolysaccharide of LPS appears to render B. japonicum more resistant to organic acid.
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